H19 inhibition orchestrates DNMT3B-dependent epigenetic reprogramming of glycolytic enzymes in the liver during diabetes

Dynamic gene expression in response to lifestyle and environmental changes is a hallmark of diabetic pathophysiology. Although altered DNA methylation is implicated during diabetes, upstream regulatory events remain poorly understood. Expression and activity of H19, DNMTs, Hk2, and Eno3 were evaluated in diabetic db/db mouse models and Hepa 1–6, HepG2 cells using qRT-PCR, Western blotting and enzyme activity assays. Functional studies were performed through siRNA knockdown and overexpression in