Binding orientation of weakly associating membrane peripheral proteins via membrane paramagnetic relaxation enhancement NMR

Cell-membrane signaling and trafficking rely on proteins that associate with lipid bilayers through dynamic, low-affinity interactions. Defining how these proteins dock onto membrane surfaces is therefore essential for understanding their function. While Neutron Reflectometry (NR) combined with molecular dynamics (MD) simulations is frequently used, complementary approaches that do not require access to large-scale neutron facilities are needed. Here, we establish membrane Paramagnetic Relaxatio