Abstract 741: Illuminate your targets: Endogenous HiBiT knock-in monoclones—inventory + rapid custom.

Abstract Background & significance — applications HiBiT is an 11-aa tag that complements LgBiT to reconstitute NanoLuc, delivering a bright, linear bioluminescent readout that scales with target protein abundance. Compared with Western blot-centric workflows, endogenous HiBiT knock-ins enable real-time, quantitative kinetics under native regulation, accelerating mechanism confirmation, potency ranking, SAR, and combination design across modalities—PROTACs, molecular glues, LYTACs, and AbTACs