Recombinant protein platform for high-throughput investigation of peptide-liposome interactions via fluorescence anisotropy depolarization

Abstract Many cytosolic proteins critical to membrane trafficking and function contain an unstructured domain that can bind to specific membranes, with a transition into an amphipathic helix induced upon membrane association. These inducible amphipathic helices often play a critical role in organelle recognition and subsequent function, but the tools used to characterize affinity towards specific membranes are low-throughput and dependent on the solubility of the inducible amphipathic helix. Her