Dynamic Phosphoproteomic Profiling Captured Early Signaling Events for Cold Acclimation in Larval Zebrafish

The acclimation at mild low temperature can improve the ability of cold tolerance and protect tissues from stress-induced injury in many species, but mechanisms triggering the signal transduction of cold acclimation remain largely unknown. Here, we deciphered the early signaling events associated with immediate temperature drops in zebrafish larvae. Early alternations in the phosphoproteome of larval zebrafish were captured during cold exposure by using an optimized sampling protocol of denaturi