Formate-driven efficient pyruvate biosynthesis in Vibrio natriegens

Formic acid (FA), a key one-carbon liquid compound derived directly from CO 2 , can serve as a dual-purpose substrate in microbial metabolism, supplying both carbon and energy. Its potential for green biomanufacturing is immense, yet its inherent toxicity and poor metabolizability to most microbes pose a major hurdle in developing efficient microbial cell factories for value-added chemical production. Building on our prior discovery of Vibrio natriegens as a naturally proficient formic acid util