Mass Spectrometry Proteomics of the Nanoparticle Corona Is Highly Dependent on Sample Preparation Protocol

It is widely established that the layers of proteins bound to the surface of nanoparticles (NPs), also known as the protein corona, critically influence their behavior in biological environments. Despite numerous proteomics studies performed in different NP-corona systems, the impact of proteomic sample preparation methods on corona profiling remains poorly understood. In this study, we systematically compared five digestion protocols, namely S-Trap^TM, iST^TM, ProteaseMAX<su