Hydropathy Landscapes of Histone–DNA Interactions in Chromatin Building Blocks

Histone-DNA interactions define the fundamental building blocks of chromatin, yet their physicochemical organization is most often interpreted through structural and electrostatic descriptors. Here, we apply the Protocol for Assigning a Residue's Character on a Hydropathy (PARCH) scale to multiple aspects of the nucleosome that are critical for DNA packaging, providing a residue-resolved, environment-dependent measure of hydrophobic and hydrophilic character. Using PARCH, we show that nucleobase