A Relatively Slow Membrane Remodeling after the Salt Induced Collapse of a Giant Unilamellar Vesicle Revealed by the Multimodal Imaging Method

To probe the detailed structural evolution of molecules in lipid membranes provides critical information for understanding the biophysics in cells. This study employed second harmonic generation (SHG) plus two-photon fluorescence (TPF) and SHG plus bright field imaging techniques to monitor the structural evolution of a giant unilamellar vesicle (GUV) composed of cardiolipin under hyperosmotic stress induced by an electrolyte (NaCl). After the contraction of the lipid vesicle upon the influence