GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming
Caroline Gravastrand·Harald Husebye·Stine Kristensen·Astrid Skjesol·Carmen Chen·Sindre Ullmann·Zunaira Iqbal·Karoline Ruud Dahlen·Kashif Rasheed·Unni Nonstad·Liv Ryan·Terje Espevik·Maria Yurchenko
Membrane trafficking through the trans-Golgi network has been shown to guide activation of the NLRP3 inflammasome. Rab11 GTPases and their effector Rab11-FIP2 regulate endosomal trafficking and retrograde transport. Here, we demonstrate that Rab11b and Rab11-FIP2 contribute to NLRP3 and pro-IL-1β stabilization during the inflammasome priming phase, which is followed by inflammasome activation. We show Rab11-FIP2 to promote TAK1 phosphorylation and TAK1-mediated activation of IKKβ, a process cont