Improvement of binding and function of a monoclonal antibody against Shigella flexneri 3a O-antigen via phage display and whole-cell in-solution panning
Nicholas L. Xerri·Hayden R. Schmidt·Mateusz Kędzior·Robert W. Kaminski·Sophia Pulido·Paul Savarino·Torrey Williams·Robert M. Gallant·Devin Sok
As rates of antimicrobial resistance (AMR) among bacterial pathogens continue to rise, the discovery and development of novel classes of therapeutics that can serve as alternatives or adjuncts to traditional small-molecule antibiotics, such as monoclonal antibodies (mAbs), is a public health priority. Some of the most promising antigen targets for antibacterial mAbs are surface polysaccharides such as O-antigen (O-Ag), a component of the lipopolysaccharide found on the outer membrane of gram-neg