Manganese (Mn) is essential for plant growth and development, yet plants in acidic and waterlogged soils are vulnerable to Mn2+ toxicity due to increased bioavailability. Metal tolerance protein 11 (MTP11) is a key Mn transporter in Arabidopsis (Arabidopsis thaliana) mediating Golgi-dependent exocytosis under high Mn stress; however, its regulatory mechanisms are thus far unknown. Here, we determined that complexes composed of calcineurin B-like proteins (CBL2/3) and CBL-interacting protein kina