Effect of tequila vinasse irrigation on soil physicochemical properties, heavy metals, and bacterial communities in soils cultivated with Agave tequilana

Tequila production yields 10–12 liters of tequila vinasses (TV) per liter of product, posing significant waste management challenges. Land application offers a cost-effective disposal method with potential fertilization benefits, though its impact on soil microbial ecology remains uncertain. In this one-year field case study in Amatitán, Jalisco, Mexico, seven TV treatments were tested: raw TV at 1, 3, 5, and 10 L/m²/month; neutralized TV at 10 L/m²/month; and raw or neutralized TV at 30 L/m² ev