High-performance thin-layer chromatographic method for simultaneous determination of some Angiotensin II Receptor Antagonists with amlodipine in spiked human plasma with UV detection

A simple, sensitive, precise, and cost-effective high-performance thin-layer chromatographic method has been developed and validated for the simultaneous determination of Amlodipine and some Angiotensin II Receptor Antagonist drugs, including Olmesartan, Telmisartan, Candesartan, Losartan, and Irbesartan, in spiked human plasma. The HPTLC analysis utilized a mobile phase consisting of toluene: ethyl acetate: methanol: acetone: acetic acid (6:1.5:1:0.5:1, v/v/v/v/v) on an aluminum-backed layer of