Dynamic matrix remodeling in boronate ester hydrogels for 3D organoid cultures
M. Neumann·T. Vermonden·D. Hène·L. van Uden·K. Schneeberger·L.J.W. van der Laan·J. Drost·G.G. Slaats·M.C. Verhaar·D. Kožinec
This study demonstrates the development of a viscoelastic hydrogel-based matrix designed to promote physiological tissue microenvironment in 3D cell cultures, utilizing hyaluronic acid-a natural constituent of the extracellular matrix-as the primary polymer. The use of dynamic covalent chemistry, specifically boronate ester bonds, allows for the creation of a tunable biomimetic material with reversible bonds conducive to cell-mediated matrix remodeling. Poly(vinyl alcohol) was employed as cross-