This study demonstrates the development of a viscoelastic hydrogel-based matrix designed to promote physiological tissue microenvironment in 3D cell cultures, utilizing hyaluronic acid-a natural constituent of the extracellular matrix-as the primary polymer. The use of dynamic covalent chemistry, specifically boronate ester bonds, allows for the creation of a tunable biomimetic material with reversible bonds conducive to cell-mediated matrix remodeling. Poly(vinyl alcohol) was employed as cross-