Surface proteins of gram-positive bacteria are key virulence factors in the human pathogen Staphylococcus aureus. Most surface proteins carry a YSIRK/G-S type signal peptide that promotes cross-wall trafficking and attachment to the septal cell wall during cell division. This study identified several new factors regulating this process through a comprehensive screen. The mutants identified here display dysregulated cell wall synthesis along with cell cycle defects. The results provide new