Ca²⁺ leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair

Endomembrane damage of intracellular vesicles triggers signals that activate membrane repair in mammalian cells to restore homeostasis. However, the signals that drive diverse membrane repair recruitment at the individual organelle level are unknown. Here by recording Ca²⁺ leakage history with a newly developed Ca²⁺ probe in human macrophages, we discovered that Ca²⁺ leakage serves as a conserved signal that triggers ATG8/LC3 lipidation after different types of sterile memb