Revealing and Exploiting the Biochemistry of O -GlcNAc through Protein Semisynthesis

O-GlcNAcylation is a dynamic posttranslational modification regulated by the enzymes O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA). It involves the attachment of N-acetylglucosamine to serine or threonine residues of proteins in the cytosol, nucleus, and mitochondria. As a dynamic and abundant modification, O-GlcNAcylation functions as a sensor of the cell's metabolic state. Fluctuations in O-GlcNAc levels of the adenosine (O-GlcNAc) sig