Enhancing cytidine biosynthesis by coupling minicell-forming morphology with biofilm-based fermentation

Cytidine is an important nucleoside in the pharmaceutical and biotechnology fields. To improve its biosynthesis efficiency, this study proposes a strategy that integrates minicell-forming recombinant strains with biofilm-based fermentation. By knocking out the key gene <i>minC</i> that regulates cell division, a cytidine-producing strain capable of stable minicell formation was constructed. In the biofilm-based fermentation system, the cytidine titer of this strain reached 1.74 g/L, representing