Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus

Large amounts of xylose mother liquor (XML) are produced as a byproduct of the xylose industry and are often treated as waste. Salidroside is a high-value glycoside with pharmaceutical and nutraceutical applications, but conventional extraction from plants cannot meet market demand and threatens the sustainability of <i>Rhodiola</i> resources. Here, XML was valorized for <i>de novo</i> salidroside production using engineered <i>Kluyveromyces marxianus</i>. The <i>KmPDC1</i> disruption promoted p