Pseudomonas aeruginosa is a highly adaptable opportunistic pathogen with a repertoire of mechanisms for surviving in diverse and often challenging environments, yet it is most studied at 37°C as the optimum temperature for growth. To better understand how this bacterium survives in the environment versus the human body, we performed transcriptomics on P. aeruginosa grown at 25°C and 37°C. At each temperature, we examined both exponential and stationary phases and determined the Las