Engineering the yeast Yarrowia lipolytica for biomanufacturing

The oleaginous yeast Yarrowia lipolytica has become a prominent cell factory for industrial biotechnology due to its robust physiology and metabolic versatility. The genetic toolkits have advanced from early approaches based on nonhomologous end joining to precise CRISPR editing and, recently, to high-throughput genome engineering. Genome-scale metabolic modeling, 'omic data, and other systems metabolic engineering approaches further accelerate the development of Y. lipolytica strains for bioman