Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica

Flavonoid C-glycosides are bioactive compounds with significant pharmaceutical potential. While biosynthesis offers a sustainable and green alternative to traditional chemical synthesis, its industrial scalability has been hindered by the poor catalytic efficiency and low thermostability of natural C-glycosyltransferases (CGTs). In this study, we report a structure-coevolution dual-guided engineering strategy to modify TcCGT1 from <i>Trollius chinensis</i>, a pivotal enzyme in flavonoid C-glycos