Single-molecule fluorescence spectroscopy of fast protein dynamics

Single-molecule experiments have become an integral part of modern structural biology. Unlike other methods, single-molecule Förster resonance energy transfer (smFRET) spectroscopy opens direct access to distance-based temporal trajectories of protein motions. Recent innovations in analysing smFRET experiments with correlation and photon-trajectory based methods have pushed the time resolution of dynamics to much faster than milliseconds. Here, we review these methods, together with their most r