Regulatory mode theory differentiates between two self-regulatory modes: locomotion mode and assessment mode. However, their effects on performance and well-being remain inconclusive. To address this gap, we conducted a meta-analysis with 52 independent samples across 43 studies (N = 15,486) to explore the relationships between regulatory modes and performance and well-being. The results indicated that locomotion mode was positively related to task performance, eudaimonic and hedonic well-being,