Integrative analysis of metagenomics and metabolomics reveals rhizospheric regulatory strategies for soybean adaptation to gradient phosphorus stress

Abstract Background and aims Soybean is sensitive to phosphorus (P) supply, but the rhizosphere ecological mechanisms underlying its low-P tolerance, particularly the synergistic regulatory network between microorganisms and metabolites, are not well understood. This study aimed to integrate metagenomics and metabolomics to reveal the rhizosphere regulatory strategies of soybean in response to graded low-P stress. Methods Three P levels were established (severe deficiency, P0; mild deficiency, P