Fragilariopsis cylindrus is a key diatom in the Southern Ocean, where low iron and manganese availability constrain primary production and biogeochemical activity. The molecular mechanisms used by polar diatoms, including F. cylindrus, to cope with trace metal limitations remain largely unexplored. Here we present phenotypic characterizations and proteomic profiles of F. cylindrus grown under controlled iron (low, medium, high) and manganese (low, high) conditions that reflect those observed in