Cell-based fluorescence and luminescence assays profit substantially from measurements performed from the bottom of the well. This reduces the distance between the cell layer and the detector, minimizing the effect of the cell culture medium.
Calcium assays (FURA-2, FLUO-3/4/8, Indo, etc.) and transcription assays (beta-lactamase, GeneBLAzer®, etc.) need sensitive bottom reading to be properly me…
