The advancement of bioink tailored for extrusion-based bioprinting (EBB) unveils the critical importance of the structure-property relationship that calls for advanced characterization techniques. Particularly for soft hydrogel in printing central nervous tissues, it is essential to ensure cell viability and differentiation with customized architecture. This study introduces a nanoengineered chitosan micelle-crosslinked hydrogel (CDP) system, achieving three distinct rheological properties by re