Insights into volatile fatty acid production and fermentation pathways of microalgae biomass combined with vinasse and glycerol
Dicla Cesário Pereira de Oliveira·Wanderli Rogério Moreira Leite·Agnes Adam Duarte Pinheiro·Mário T. Kato·Lourdinha Florencio·Federico Battista·Francyelle Karolynne Vieira Machado
Abstract The fermentation of industrial wastes derived from water recovery (pretreated microalgae biomass, PTMB), biodiesel (glycerol, GLY), and ethanol (vinasse, VIN)) is a promising sustainable alternative for producing volatile fatty acids (VFA). In this study, controlled fermentation experiments were performed using PTMB-VIN and PTMB-GLY mixtures with varying substrate concentrations and retention times to optimize the VFA yield and production efficiency. Acid fermentation was optimized at a