Proactive surveillance of foodborne bioactives by integrated dietary exposure and urinary excretion assessment

Unintentional ingestion of pharmacologically-active substances through foods and dietary supplements presents growing challenges for food safety and anti-doping regulation. We developed a high-throughput LC–MS/MS platform to simultaneously screen 331 compounds and quantify 214 analytes, with matrix-matched validation in pork, oat, and beverage. The method showed applicability across ten food types and was applied to seventy-eight commercial products, revealing frequent detection of bioactives su