Tryptophan (Trp) is an essential chiral amino acid that plays a fundamental role in numerous physiological and neurological processes. It serves as a key compound for the synthesis of biologically significant metabolites, including kynurenine (Kyn), 5-hydroxytryptophan (5-HTP), and serotonin (5-HT); each of which contributes to distinct yet interconnected biochemical functions. Except 5-HT, the other two tryptophan-related metabolites are chiral molecules. In this study, the simultaneous separat