De novo enzyme design: Controlling structure to design function

The ultimate mission of de novo enzyme design methodology is to develop strategies that produce new-to-nature enzymes that match the efficiency and versatility of natural ones. Until recent years, design methods yielded enzymes with low catalytic efficiencies even for simple reactions, underscoring the need for tighter control over backbone structure and active-site preorganization. Two approaches have recently emerged to address these problems: artificial intelligence-driven design of de novo f