Molecular beacon strategies that control the generation of singlet oxygen via intramolecular energy transfer between a ruthenium photosensitizer and COT quencher on oligodeoxynucleotides
Sae Harada·Kazuhito Tanabe·Kazuki Iwasawa·Yasuko Osakada·Kiyohiko Kawai·Tatsuya Nishihara·Daisuke Mochizuki
Abstract A molecular beacon-based platform that enables the switchable, DNA-structure-dependent generation of singlet oxygen ( 1O2) was designed. We prepared stem-and-loop structured oligodeoxynucleotides with an excellent photosensitizer, ruthenium complex, and cyclooctatetraene quencher attached at each strand end. The stem-and-loop structured oligodeoxynucleotides suppressed the photosensitized generation of 1O2 from the ruthenium photosensitizer owing to intramolecular quenching by the quenc